| 1 | BO | PT Bouraq Indonesia Airlines | ICAO: BOU, Callsign: Bouraq, IATA Prefix: 666, | RI | AVCODES |
| 2 | BO | Air Columbus | ICAO: CNB, Callsign: Air Columbus, Ceased operations | P | AVCODES |
| 3 | BO | Delta Air Regionalflugverkhr GmbH | ICAO: DEL, Callsign: Bravo Oscar, ICAO callsign changed to Delta Air | D | AVCODES |
| 4 | BO | AirBridge Cargo Airlines Limited | ICAO: ABW, Callsign: AirBridge Cargo, IATA Prefix: 580, IATA designator changed to RU | RUS | AVCODES |
| 5 | BO | Delta Air Regionalflugverkhr GmbH | ICAO: DEL, Callsign: Delta Air, IATA Designator changed to DI. ICAO callsign changed from Bravo Oscar | D | AVCODES |
| 6 | BO | BB Airways Pvt. Limited | ICAO: BBW, Callsign: BeeBee Airways, IATA Prefix: 505, Founded in January 2012. Commenced operations on 13 September 2012. Ceased operations in July 2013. | NEP | AVCODES |
| 7 | BO | Bláfugl ehf. d/b/a Bluebird Cargo Ltd. | ICAO: BBD, Callsign: Blue Cargo, IATA Prefix: 290, Established in 1999. Name changed to Bl�fugl ehf d/b/a bluebird NORDIC. IATA designator changed from BF. | IS | AVCODES |
| 8 | BO | Bláfugl ehf. d/b/a bluebird NORDIC | ICAO: BBD, Callsign: Blue Cargo, IATA Prefix: 290, Established in 1999. Name changed from Bl�fugl ehf d/b/a Bluebird Cargo Ltd. IATA designator changed from BF. | IS | AVCODES |
| 9 | BO | CSX Transportation, Inc. | added 10/1987; 1/1994-7/2005 | | NAKINA |
| 10 | BO | Baltimore & Ohio R.R. Co. | 1/1975-1/1980; eliminated 7/1987 | | NAKINA |
| 11 | BO | The Chesapeake and Ohio Ry. Co. | added 7/1987; eliminated 10/1987 | | NAKINA |
| 12 | BO | Baltimore & Ohio R.R. Co. | 1/75-1/80; eliminated 7/87 | | NRHS |
| 13 | BO | The Chesapeake and Ohio Ry. Co. | added 7/87; eliminated 10/87 | | NRHS |
| 14 | BO | CSX Transportation, Inc. | added 10/87; 1/94-7/2000 | | NRHS |
| 15 | BO | CSX [BALTIMORE & OHIO RR] | | | PWRC |
| 16 | BO | Baltimore and Ohio Railroad (CSX Transportation) | | | RSRV |
| 17 | BO | Baltimore and Ohio | | USA | TRAFFIC |
| 18 | BOA | Boniair D.O.O.E.L. | Callsign: Kumanovo, Founded in 1996. | MK | AVCODES |
| 19 | BOA | Boniair | | | ICAO1 |
| 20 | BOAB | BOALS, BERNARD B | | | SCAC3 |
| 21 | BOAC | British Overseas Airways Corporation | | GB | TRAFFIC |
| 22 | BOAD | BROAD TOP MINING & TRUCKING COMPANY | | | SCAC3 |
| 23 | BOAE | BLUE OX EQUIPMENT INC | | | SCAC4 |
| 24 | BOAG | BOAG WAREHOUSE COMPANY | | | SCAC3 |
| 25 | BOAG | Borner Transport und Lagerhaus AG | | CH | VKM |
| 26 | BOAI | BOWKER, GRANT | | | SCAC4 |
| 27 | BOAJ | BONNER, A JAMES | | | SCAC3 |
| 28 | BOAJ | BOUNDARY ELECTRIC (1985) LTD | | | SCAC4 |
| 29 | BOAK | BROOKS AERO INC | | | SCAC3 |
| 30 | BOAM | BOOTH AMERICAN SHIPPING CORPORATION | | | SCAC3 |
| 31 | BOAM | BOYZ TRANSPORTATION SERVICES LLC | | | SCAC4 |
| 32 | BOAN | BOWEN DELIVERY SERVICE INC | | | SCAC4 |
| 33 | BOAQ | BOBS APPLICANCE DELIVERY SERVICE | | | SCAC3 |
| 34 | BOAR | BORING, AL TRUCKING | | | SCAC3 |
| 35 | BOAR | BAZAAR DE MEXICO INC | | | SCAC4 |
| 36 | BOAS | BOLDUC & SONS INC | | | SCAC3 |
| 37 | BOAS | BOLDUC AND SONS INC | | | SCAC4 |
| 38 | BOAY | BOWEN, ALBERT E INC | | | SCAC3 |
| 39 | BOAY | BR MOVERS | | | SCAC4 |
| 40 | BOB | Jet Club Ltd. | Callsign: Bob Jet, | PL | AVCODES |
| 41 | BOB | Bonair Business Charter GmbH | Callsign: Bob, ICAO callsign changed to Twister | D | AVCODES |
| 42 | BOB | Bonair Business Charter GmbH | Callsign: Twister, ICAO callsign changed from Bob | D | AVCODES |
| 43 | BOB | MLP Aviation | Callsign: Bobfly, | GB | AVCODES |
| 44 | BOB | Backbone Aviation A/S | Callsign: Backbone, | DK | AVCODES |
| 45 | BOB | Bayerische Oberlandbahn GmbH | Holzkirchen (auch BayOB, Connex-Gruppe > Veolia Verkehr) | D | privat-bahn.de |
| 46 | BOB | Bodensee-Oberschwaben Bahn GmbH | Friedrichshafen | D | privat-bahn.de |
| 47 | BOB | Bayerische Oberlandbahn | | D | TRAFFIC |
| 48 | BOB | Berner Oberland Bahnen | | CH | VKM |
| 49 | BOBA | BOBCAT COMPANY | | | SCAC4 |
| 50 | BOBB | BOBS BROKERAGE INC | | | SCAC4 |
| 51 | BOBC | BOUCHER, L S EXPRESS INC | | | SCAC4 |
| 52 | BOBD | BOARDMAN, BUDDY TRUCKING | | | SCAC3 |
| 53 | BOBE | BOBS EXPRESS LINE | | | SCAC3 |
| 54 | BOBF | BOBS TRANSFER INC | | | SCAC3 |
| 55 | BOBFN | Bodensee-Oberschwaben-Bahn | | DE | VKM |
| 56 | BOBG | BOBS TRUCKING | | | SCAC3 |
| 57 | BOBG | BOBS GARAGE INC | | | SCAC4 |
| 58 | BOBH | BOBS HI DISTRIBUTORS | | | SCAC3 |
| 59 | BOBH | BLOOM DEPOT INC | | | SCAC4 |
| 60 | BOBI | BOBBITT TRUCKING | | | SCAC4 |
| 61 | BOBJ | BUTTERBAUGH OIL CO INC | | | SCAC4 |
| 62 | BOBK | BOYD, BOB TRUCKING INC | | | SCAC3 |
| 63 | BOBN | BOBS TRUCKING | | | SCAC3 |
| 64 | BOBN | BANNER TRANSPORT INC | | | SCAC4 |
| 65 | BOBO | BOBS DELIVERY SERVICE | | | SCAC3 |
| 66 | BOBO | BOB-O EXPRESS | | | SCAC4 |
| 67 | BOBP | BOBS TRANSFER & STORAGE | | | SCAC3 |
| 68 | BOBP | BYRD TRANSPORT INC | | | SCAC4 |
| 69 | BOBQ | BOBB, MICHAEL J INC | | | SCAC3 |
| 70 | BOBR | BORDER BROKERAGE INC | | | SCAC3 |
| 71 | BOBR | BUSINESS SOLUTIONS INTERNATIONAL IN | | | SCAC4 |
| 72 | BOBT | B & B TRAVEL INC | | | SCAC3 |
| 73 | BOBU | LANXESS SOLUTIONS UK LTD | | GB | BIC |
| 74 | BOBU | GREAT LAKES UK LTD | | | SCAC4 |
| 75 | BOBV | BOBO TRANSPORT | | | SCAC4 |
| 76 | BOBX | Freeman Gas of N.C., Inc. | added 1/1971; 1/1975-7/2000; eliminated 4/2003; 7/2005 (not listed) | | NAKINA |
| 77 | BOBX | Freeman Gas of N.C., Inc. | added 1/71; 1/75-7/2000 | | NRHS |
| 78 | BOBX | FREEMAN GAS OF NORTH CAROLINA | | | PWRC |
| 79 | BOBX | Freeman Gas of N.C. | | | RSRV |
| 80 | BOBX | FREEMAN GAS | | | SCAC4 |
| 81 | BOBY | BOBBYS TOURS | | | SCAC3 |
| 82 | BOBY | Bayerische Oberlandbahn | | DE | VKM |
| 83 | BOC | Aerobona, S.A. de C.V. | Callsign: Aerobona, | MEX | AVCODES |
| 84 | BOC | Aerobona | | | ICAO1 |
| 85 | BOC | BORTNER BUS COMPANY | | | PWRC |
| 86 | BOC | BORTNER BUS COMPANY | | | SCAC4 |
| 87 | BOCA | BOSTON COACH CORP | | | SCAC3 |
| 88 | BOCB | BELLMAN OIL CO INC | | | SCAC4 |
| 89 | BOCC | BOCCHI, A TRUCKING, INC | | | SCAC3 |
| 90 | BOCD | BOSTON CARRIERS, INC | | | SCAC3 |
| 91 | BOCD | BROCK, T INC | | | SCAC4 |
| 92 | BOCE | BOYCE TRUCKING | | | SCAC3 |
| 93 | BOCF | BOCIMAR SA NV | | | SCAC3 |
| 94 | BOCH | BORCHARDT, ELMER INC | | | SCAC3 |
| 95 | BOCH | BOUCHARD, STEPHANE TRANSPORT | | | SCAC4 |
| 96 | BOCH | Bochum-Gelsenkirchener Straßenbahnen AG | Real: BOGESTRA, City: Bochum | D | TRAFFIC |
| 97 | BOCI | BOSLEY OIL CO INC | | | SCAC4 |
| 98 | BOCJ | BOARD OF COUNTY COMMISSIONERS OF PA | | | SCAC4 |
| 99 | BOCK | BOCK TRANSFER & STORAGE CO | | | SCAC3 |
| 100 | BOCK | BAUMGART OIL COMPANY LLC | | | SCAC4 |
