| 1 | EF | Far Eastern Air Transport Corp. | ICAO: FEA, Callsign: Far Eastern, IATA Prefix: 265, Ceased Operations on 13 May 2008. | RC | AVCODES |
| 2 | EF | Strategic Airlines SAS | ICAO: STZ, Callsign: French Bird, AOC revoked/not renewed | F | AVCODES |
| 3 | EF | Eznis Airways | ICAO: EZA, Callsign: Eznis, IATA Prefix: 646, Ceased operations on 22 May 2014. IATA designator changed from O7. IATA designator previously changed from O7 | MGL | AVCODES |
| 4 | EF | Equa2C t/a Fly Caminter | | CAM | AVCODES |
| 5 | EFA | Euro First Air, S.A. | Callsign: Cargocanarias, | E | AVCODES |
| 6 | EFA | Express Freighters Australia Pty Limited | IATA: QE, Callsign: Express Freight, Operates as QANTASFREIGHT. | AUS | AVCODES |
| 7 | EFA | Far Eastern Air Transport | IATA: EF | | ICAO1 |
| 8 | EFA | Estrada de Ferro Araraquarense | | BR | TRAFFIC |
| 9 | EFAR | EASTERDAY FARMS | | | SCAC4 |
| 10 | EFAS | ENRIGHT FARMS | | | SCAC4 |
| 11 | EFAU | CEFA SAS | | | SCAC4 |
| 12 | EFB | Emerald Jets S.A.L. | Callsign: Emerald Jets, | RL | AVCODES |
| 13 | EFB | Eisenbahnfreunde Betzdorf e.V. | Siegen | D | privat-bahn.de |
| 14 | EFB | Eisenbahnfreunde Breisgau e.V. | Emmendingen | D | privat-bahn.de |
| 15 | EFB | Eisenbahnfreunde Breisgau e.V. | | DE | VKM |
| 16 | EFBI | EXPORT FREIGHT & BROKER INC | | | SCAC4 |
| 17 | EFBK | EXPRESS FREIGHT BROKERAGE | | | SCAC4 |
| 18 | EFBS | EASTERN FLAT BED SYSTEMS INC | | | SCAC3 |
| 19 | EFBS | Eisenbahnfreunde Betzdorf e.V | | DE | VKM |
| 20 | EFC | Emirates Flight Training Academy | Callsign: Al Wasel, | UAE | AVCODES |
| 21 | EFC | Air Mana | | | ICAO1 |
| 22 | EFC | Eisenbahnfreunde Chemnitztal e.V | | DE | VKM |
| 23 | EFCA | E-FREIGHT COURIER LLC | | | SCAC4 |
| 24 | EFCB | Estrada de Ferro Central do Brasil / Central Brazilian Railway | | BR | TRAFFIC |
| 25 | EFCI | EXECUTIVE FREIGHT CONSOLIDATORS | | | SCAC3 |
| 26 | EFCN | EFFICIENT SERVICES INC | | | SCAC3 |
| 27 | EFCN | EASY-FLO CENTRAL VACUUMS LTD | | | SCAC4 |
| 28 | EFCS | EMPIRE TRANSPORTATION LTD | | | SCAC3 |
| 29 | EFCS | EMPIRE TRANSPORTATION LTD | | | SCAC4 |
| 30 | EFCU | FINSTERWALDER CONTAINER GMBH | | D | BIC |
| 31 | EFCU | FINSTERWALDER CONTAINER GMBH | | | SCAC4 |
| 32 | EFCX | Evergreen Freight Car Corp. | added 10/1966; 10/1966-1/1980; eliminated 10/1980 | | NAKINA |
| 33 | EFCX | Evergreen Freight Car Corp. | added 10/66; 10/66-1/80; eliminated 10/80 | | NRHS |
| 34 | EFCX | EVERGREEN FREIGHT CAR CORP. | | | PWRC |
| 35 | EFD | EFD Eisele Flugdienst GmbH | Callsign: Ever Flight, | D | AVCODES |
| 36 | EFD | EFD Eisele Flugdienst | | | ICAO1 |
| 37 | EFDL | ER FOURNIER DESIGN LTD | | | SCAC4 |
| 38 | EFDT | ENFIELD TRANSPORTATION INC | | | SCAC4 |
| 39 | EFE | Ermolino Flying Test Research Enterprise | Callsign: Ermolino Air, | RUS | AVCODES |
| 40 | EFE | E-Freight International Ltd | Callsign: Efreight Sudan, | SUD | AVCODES |
| 41 | EFE | Empresa de los Ferrocarriles del Estado | | RCH | TRAFFIC |
| 42 | EFEA | Empresa Ferrocarriles del Estado Argentino | | RA | TRAFFIC |
| 43 | EFEF | ESMERALDA, F ENTERPRISES | | | SCAC4 |
| 44 | EFEI | EFFICIENCY ENTERPRISES OF TAMPA LLC | | | SCAC4 |
| 45 | EFEQ | EFFINGHAM EQUITY | | | SCAC3 |
| 46 | EFEQ | EFFINGHAM EQUITY | | | SCAC4 |
| 47 | EFES | EFICAS TRANSFER SA DE CV | | | SCAC4 |
| 48 | EFET | E F E TRUCKING | | | SCAC3 |
| 49 | EFEU | EVER FORTUNE ENTERPRISE (H.K.) CO., LTD | | HK | BIC |
| 50 | EFF | Westair Aviation Limited | Callsign: Emerald, | IRL | AVCODES |
| 51 | EFF | Emerald Air (Pan Am Express) | Callsign: Emerald, | USA | AVCODES |
| 52 | EFFE | EFFICIENCY ENTERPRISES INC | | | SCAC3 |
| 53 | EFFI | EASTERN FREIGHT FORWARDERS INC | | | SCAC4 |
| 54 | EFFIAN | Effian Bus Company | | USA | TRAFFIC |
| 55 | EFG | Elifriulia | Callsign: Elifriulia, | I | AVCODES |
| 56 | EFG | Elifriulia | | | ICAO1 |
| 57 | EFG | Eisenbahnfreunde Grenzland e.V. | | DE | VKM |
| 58 | EFGI | EAGLE FREIGHT INC | | | SCAC4 |
| 59 | EFGS | ENERGY FREIGHT SYSTEMS CORPORATION | | | SCAC4 |
| 60 | EFGU | ELECTRONIC FUOROCARBONS, LLC | | USA | BIC |
| 61 | EFGU | Electronic Fuorocarbons | | | RSRV |
| 62 | EFH | Eisenbahnfreunde Hegau Singen e.V. | Singen | D | privat-bahn.de |
| 63 | EFHI | EFHI COMPANIA NAVIERA SA | | | SCAC3 |
| 64 | EFHI | EXPRESS FREIGHT HAULERS INC | | | SCAC4 |
| 65 | EFHL | EAST FLORIDA HAULING, INC | | | SCAC3 |
| 66 | EFHL | EAST FLORIDA HAULING INC | | | SCAC4 |
| 67 | EFIC | EXPEDITED FREIGHT INC | | | SCAC4 |
| 68 | EFID | ECO FIRST INC | | | SCAC4 |
| 69 | EFIF | EVERYTHING FITZ | | | SCAC4 |
| 70 | EFIL | EXPRESS FREIGHT INTERNATIONAL INC | | | SCAC4 |
| 71 | EFIN | EXPRESS FREIGHT INC | | | SCAC3 |
| 72 | EFIQ | ENERGY FABRICATION INC | | | SCAC4 |
| 73 | EFIV | EFI LOGISTICS INC | | | SCAC4 |
| 74 | EFK | Eisenbahnfreunde Kraichgau e.V. | Sinsheim | D | privat-bahn.de |
| 75 | EFK | Eisenbahnfreunde Klingental | Klingental | D | privat-bahn.de |
| 76 | EFK | Erich Fahl GmbH & CoKG | | DE | VKM |
| 77 | EFKA | EFKA PLASTIC CORP | | | SCAC3 |
| 78 | EFL | Eagle Air Ltd. | IATA: EY, Callsign: Flying Eagle, Designator EY* reinstated by IATA | EAT | AVCODES |
| 79 | EFL | Estrada de Ferro Leopoldina | | BR | TRAFFIC |
| 80 | EFLC | ERICKSON FUEL CO INC | | | SCAC4 |
| 81 | EFLE | EAGLE FREIGHT | | | SCAC4 |
| 82 | EFLH | EAST FLORIDA HAULING INC | | | SCAC3 |
| 83 | EFLI | EMPIRE FREIGHT LOGISTICS LLC | | | SCAC4 |
| 84 | EFLL | EDSON FREIGHT LINES (1971) LTD | | | SCAC4 |
| 85 | EFLN | E-FORCE LOGISTICS INC | | | SCAC4 |
| 86 | EFLQ | EXTREME FEDERAL LLC | | | SCAC4 |
| 87 | EFLT | E. F. L. TRANSPORTATION, INC | | | SCAC3 |
| 88 | EFLT | E F L TRANSPORTATION | | | SCAC4 |
| 89 | EFLW | ENERFLOW INDUSTRIES INC | | | SCAC4 |
| 90 | EFLY | ELMERS FLIGHTING SUPPLY INC | | | SCAC4 |
| 91 | EFMG | ESTEVEZ FLOWERS, MIGUEL | | | SCAC4 |
| 92 | EFMH | Eisenbahnfreunde Mittelholstein e.V. | Kleinkummerfeld | D | privat-bahn.de |
| 93 | EFML | EASTERN FARM MACHINERY LTD | | | SCAC4 |
| 94 | EFMN | ELITE FREIGHT MANAGEMENT INC | | | SCAC4 |
| 95 | EFMT | EFM TRANSPORTATION INC | | | SCAC4 |
| 96 | EFNL | EVERETT FUEL & LUMBER DISTRIBUTORS | | | SCAC3 |
| 97 | EFNL | EVERETT FUEL & LUMBER DISTRIBUTORS | | | SCAC4 |
| 98 | EFNOB | Estrada de Ferro Noroeste do Brasil | Real: NOB | BR | TRAFFIC |
| 99 | EFO | Estop Estudios de Fotografia, S.A. | | E | AVCODES |
| 100 | EFO | Eisenbahnfreunde Flügelrad Oberberg e.V. | Dieringhausen | D | privat-bahn.de |
