| 1 | 5A | Alpine Aviation, Inc. | ICAO: AIP, Callsign: Alpine Air, | USA | AVCODES |
| 2 | 5A | Air Atlanta Europe Ltd. | ICAO: EUK, Callsign: Snowbird, Merged into Excel Airways on 01 May 2006. AOC revoked on 08 November 2006. | GB | AVCODES |
| 3 | 5A | STP Airways | ICAO: STP, IATA designator changed to 8F* | STP | AVCODES |
| 4 | 5B | Euro-Asia International, JSC | ICAO: EAK, Callsign: Eakaz, | KZ | AVCODES |
| 5 | 5B | Trans International Express | ICAO: BAP, Callsign: Big Apple, Designator 5B recalled by IATA. Name changed from Tie Aviation | USA | AVCODES |
| 6 | 5B | Tie Aviation | ICAO: BAP, Callsign: Big Apple, Name changed to Trans International Express | USA | AVCODES |
| 7 | 5B | Gulf Coast Airways, Inc. | | USA | AVCODES |
| 8 | 5B | Bassaka Air Limited d/b/a Bassaka Air | ICAO: BSX, Callsign: Bassaka, IATA Prefix: 590, Founded in 2013. Commenced operations on 01 December 2014. | K | AVCODES |
| 9 | 5C | Air Tahoma, Inc | ICAO: HMA, Callsign: Tahoma, Founded in 1995. Ceased operations during 2009. | USA | AVCODES |
| 10 | 5C | Cavei Avir Lemitanim t/a Cargo Air Lines | ICAO: ICL, Callsign: Cal, IATA Prefix: 700, Name changed to Challenge Airlines (IL) Ltd. | IL | AVCODES |
| 11 | 5C | Travelair d/b/a Nature Air | ICAO: NRR, Callsign: Natureair, Operations were suspended by Costa Rica CAA on 12 January 2018. | CR | AVCODES |
| 12 | 5C | Challenge Airlines (IL) Ltd. | ICAO: ICL, Callsign: Cal, IATA Prefix: 700, Name changed from Cavei Avir Lemitanim t/a Cargo Air Lines. | IL | AVCODES |
| 13 | 5D | Dutchbird | ICAO: DBR, Callsign: Dutchbird, | NL | AVCODES |
| 14 | 5D | Air Company Donbass Airlines | ICAO: UDD, Callsign: Donbass, IATA designator changed from 7D | UA | AVCODES |
| 15 | 5D | Aerolitoral S.A. de C.V. | ICAO: SLI, Callsign: Costera, IATA Prefix: 642, Name changed to Aerolitoral, S.A. de C.V., d/b/a Aerom�xico Connect. Designator 5D reinstated by IATA | MEX | AVCODES |
| 16 | 5D | Air Company Donbass Airlines | ICAO: UDD, Callsign: Donbass, Designator 5D recalled by IATA. IATA designator changed from 7D | UA | AVCODES |
| 17 | 5D | V BIRD Airlines Netherlands B.V. | ICAO: VBA, Callsign: VeeBee, IATA designator changed to VX | NL | AVCODES |
| 18 | 5D | Aerolitoral, S.A. de C.V., d/b/a Aeroméxico Connect | ICAO: SLI, Callsign: Costera, IATA Prefix: 642, Name changed from Aerolitoral S.A. de C.V. Designator 5D reinstated by IATA | MEX | AVCODES |
| 19 | 5E | Business Aviation Services Eindhoven BV d/b/a Base Airlines | ICAO: BRO, Callsign: CoastRider, IATA Prefix: 228, Ceased operations on 06 April 2001. | NL | AVCODES |
| 20 | 5E | Equaflight Service | ICAO: EKA, Callsign: Equaflight, Designator 8H* recalled by IATA. Designator 8H* reinstated by IATA | RCB | AVCODES |
| 21 | 5E | Alfa Airlines SD Co. Ltd. | ICAO: AAJ, Callsign: Alfa Sudan, | SUD | AVCODES |
| 22 | 5E | Siam General Aviation Company Limited d/b/a Nok Mini Airlines | ICAO: SGN, Callsign: Siam, Ceased Operations during 2014. | T | AVCODES |
| 23 | 5E | East Coast Flight Services, Inc. | | USA | AVCODES |
| 24 | 5F | Arctic Circle Air Service, Inc. | ICAO: CIR, Callsign: Air Arctic, | USA | AVCODES |
| 25 | 5F | Tunca Havacilik A.S. Bestair | ICAO: FBA, ICAO designator changed to BST | TR | AVCODES |
| 26 | 5F | Tunca Havacilik A.S. Bestair | ICAO: BST, Callsign: Barbie, IATA Prefix: 759, Ceased operations in October 2009. Started operations on 17 June 2006. | TR | AVCODES |
| 27 | 5F | Pan African Airways Ltd | ICAO: ODM, Callsign: Jetafrica, | EAK | AVCODES |
| 28 | 5F | FlyOne Airlines S.R.L. | ICAO: FIA, Callsign: Fia Airlines, | MD | AVCODES |
| 29 | 5G | Queen Air | ICAO: QNA, Callsign: Queen Air, IATA designator changed from 7Q* | DOM | AVCODES |
| 30 | 5G | Skyservice Airlines, Inc. | ICAO: SSV, Callsign: Skytour, IATA Prefix: 884, Ceased operations on 31 March 2010. IATA Designator changed from 6J*. Name changed from Skyservice Airlines Inc. d/b/a Skyservice Roots Air | CDN | AVCODES |
| 31 | 5G | Aircompany "Global Air" Ltd. | ICAO: GGB, Callsign: Globalair, IATA Prefix: 166, Designator 5G* recalled by IATA. | GE | AVCODES |
| 32 | 5G | 540 Ghana Ltd. | ICAO: FOX, | GH | AVCODES |
| 33 | 5G | Aero Cuahonte, S.A. de C.V. | | MEX | AVCODES |
| 34 | 5G | Shirak Avia LLC | ICAO: SHS, Callsign: Shirak, IATA Prefix: 740, | AM | AVCODES |
| 35 | 5H | Edelweiss Air AG | ICAO: EDW, Callsign: Edelweiss, IATA desigantor changed to 8R*. IATA desigantor changed from 1I | CH | AVCODES |
| 36 | 5H | Star Air | ICAO: STZ, IATA Prefix: 301, ICAO designator changed to STQ | RI | AVCODES |
| 37 | 5H | Heli-Trans AS | ICAO: HTA, Callsign: Scanbird, Designator 5H recalled by IATA | N | AVCODES |
| 38 | 5H | Star Air | ICAO: STQ, Callsign: Stera, IATA Prefix: 301, Designator 5H recalled by IATA. ICAO designator changed from STZ | RI | AVCODES |
| 39 | 5H | Five Fourty Aviation Limited | ICAO: FFV, Callsign: Swift Tango, IATA Prefix: 540, IATA Accounting and Prefix codes recalled. | EAK | AVCODES |
| 40 | 5H | Trans Executive Airlines of Hawaii t/a transair Express | ICAO: MUI, Callsign: Maui, Operates transair Express SD360 Fleet. Designator 5H recalled by IATA | USA | AVCODES |
| 41 | 5H | Five Fourty Aviation Limited | ICAO: FFV, Callsign: Swift Tango, IATA Accounting and Prefix codes recalled. | EAK | AVCODES |
| 42 | 5I | Trans Air Georgia | ICAO: GRG, IATA Prefix: 936, Name changed to Air G | GE | AVCODES |
| 43 | 5I | Air G | ICAO: GRG, IATA Prefix: 936, Designator 5I recalled by IATA. Name changed from Trans Air Georgia | GE | AVCODES |
| 44 | 5I | ALSA Grupo S.L.U. | | E | AVCODES |
| 45 | 5J | CEBU Pacific Air | ICAO: CEB, Callsign: Cebu Air, IATA Prefix: 203, Name changed to Cebu Air, Inc. d/b/a Cebu Pacific Air. ICAO designator changed from CPI | RP | AVCODES |
| 46 | 5J | Private Jet Expeditions, Inc. | ICAO: PJE, Callsign: Pee Jay, Commenced Operations in 1989. Filed for Chapter 11 Bankruptcy protection 1994. Ceased operations in March 1995. | USA | AVCODES |
| 47 | 5J | CEBU Pacific Air | ICAO: CPI, Callsign: Cebu Air, IATA Prefix: 203, ICAO designator changed to CEB | RP | AVCODES |
| 48 | 5J | Cebu Air, Inc. d/b/a Cebu Pacific Air | ICAO: CEB, Callsign: Cebu Air, IATA Prefix: 203, Name changed CEBU Pacific Air. ICAO designator changed from CPI | RP | AVCODES |
| 49 | 5K | State Air Company Odessa Airlines | ICAO: ODS, Callsign: Odessa Air, IATA Prefix: 654, Founded in 1996. | UA | AVCODES |
| 50 | 5K | HiFly Transportes Aereos, S.A. | ICAO: HFY, Callsign: Sky Flyer, IATA Prefix: 102, Name changed from Air Luxor | P | AVCODES |
| 51 | 5K | Sky King, Inc. | ICAO: SGB, Callsign: Songbird, Ceased operations on 26 January 2014. Designator F3* recalled by IATA. Name changed from Sky King, Inc. d/b/a Fly First Class | USA | AVCODES |
| 52 | 5K | HiFly Transportes Aereos, S.A. | ICAO: HFY, Callsign: Sky Flyer, IATA Prefix: 026, Name changed to Springjet, S.A. t/a HiFly w.e.f. 25 August 2021. Name changed from Air Luxor | P | AVCODES |
| 53 | 5K | Springjet, S.A. t/a HiFly | ICAO: HFY, Callsign: Sky Flyer, IATA Prefix: 026, Name changed from HiFly Transportes Aereos, S.A. w.e.f. 25 August 2021. Name changed from Air Luxor | P | AVCODES |
| 54 | 5L | Compania Boliviana de Transporte Aereo Privado Aerosur, S.A. | ICAO: RSU, Callsign: Asur, IATA Prefix: 275, Founded in 1992. Ceased operations on 17 May 2012. AOC revoked on 20 July 2012. | BOL | AVCODES |
| 55 | 5L | Fly Salone Airways Ltd | | WAL | AVCODES |
| 56 | 5L | Transportes Aereos Costarricenses | ICAO: TIC, Callsign: Tac Airlines, | CR | AVCODES |
| 57 | 5M | Joint Stock Company SIAT (Sibaviatrans) | ICAO: SIB, Callsign: Sibavia, IATA Prefix: 842, Suspended operations at the end of September 2008 due to the Air Union collapse. | RUS | AVCODES |
| 58 | 5M | Murray Air, Inc. | ICAO: MUA, Callsign: Murray Air, IATA Prefix: 416, Name changed to National Air Cargo Group, Inc. d/b/a National Airlines. Name changed from Murray Aviation, Inc. | USA | AVCODES |
| 59 | 5M | Montserrat Airways Ltd. t/a FlyMontserrat | ICAO: MNT, Callsign: Montserrat, Name changed from Montserrat Airways Ltd. | MS | AVCODES |
| 60 | 5M | Mega Global Air Services (Maldives) Pvt. Ltd. t/a MEGA Maldives Airlines | ICAO: MEG, Callsign: Sandbar, Founded on 23 February 2010. AOC granted on 22 December 2010. Commenced operations on 23 December 2010. IATA Designator changed to LV | MV | AVCODES |
| 61 | 5M | Hi Fly Ltd. | ICAO: HFM, Callsign: Moonraker, IATA Designator 5M* recalled. | M | AVCODES |
| 62 | 5M | Mediterranean Aviation Operations Company Ltd. | ICAO: MDO, Callsign: Kuda, Name changed to Mediterranean Aviation Operations Company Ltd. d/b/a MedOps | M | AVCODES |
| 63 | 5M | Mediterranean Aviation Operations Company Ltd. d/b/a MedOps | ICAO: MDO, Callsign: Kuda, Name changed from Mediterranean Aviation Operations Company Ltd. | M | AVCODES |
| 64 | 5M | Mel Air Limited | ICAO: MDO, Callsign: Kuda, Name changed from Mediterranean Aviation Operations Company Ltd. d/b/a MedOps and previously from Mediterranean Aviation Operations Company Ltd. | M | AVCODES |
| 65 | 5N | Arkhangelsk Airlines | ICAO: OAO, Callsign: Dvina, IATA Prefix: 316, Name changed to Aeroflot - Nord. ICAO codes changed to AUL. IATA Designator changed from 5N* | RUS | AVCODES |
| 66 | 5N | Arkhangelsk Airlines | ICAO: OAO, Callsign: Dvina, IATA Designator changed to 5N | RUS | AVCODES |
| 67 | 5N | Joint Stock Company Nordavia Region Airlines | ICAO: AUL, Callsign: Dvina, IATA Prefix: 316, To be rebranded as SmartAvia w.e.f. October 2017. Name changed from Aeroflot - Nord w.e.f. 01 December 2009. Name changed from Arkhangelsk Airlines. ICAO codes changed from OAO. IATA Designator changed from 5N* | RUS | AVCODES |
| 68 | 5N | Joint Stock Company Smartavia Airlines | ICAO: AUL, Callsign: Dvina, IATA Prefix: 316, Name changed from Joint Stock Company Nordavia Region Airlines. Name changed from Arkhangelsk Airlines. ICAO codes changed from OAO. IATA Designator changed from 5N* | RUS | AVCODES |
| 69 | 5O | Europe Airpost | ICAO: FPO, Callsign: French Post, IATA Prefix: 558, Name changed to ASL Airlines France S.A. Name change from Societe Financiereale (S.F.A.) | F | AVCODES |
| 70 | 5O | ASL Airlines France S.A. | ICAO: FPO, Callsign: French Post, IATA Prefix: 558, Name changed from Europe Airpost. Name change from Societe Financiereale (S.F.A.) | F | AVCODES |
| 71 | 5P | Ptarmigan Airways Ltd. | ICAO: PTA, Callsign: Ptarmigan, Designator 5P recalled by IATA. Bought and merged in First Air during 1995. | CDN | AVCODES |
| 72 | 5P | Pena Transportes Aereos, S.A. | ICAO: PEP, Callsign: Aero Pena, IATA Prefix: 308, Founded and Commenced operations in 1995. Name changed to PENTA - Pena Transportes Aereos S.A. IATA designator reinstated | BR | AVCODES |
| 73 | 5P | Sky Europe Airlines Hungary | ICAO: HSK, Callsign: Matra, Designator 5P recalled by IATA | H | AVCODES |
| 74 | 5P | Small Planet Airlines GmbH | ICAO: LLX, Callsign: Germanjet, Ceased operations on 31 October 2018. | D | AVCODES |
| 75 | 5P | ACM Air Charter GmbH | ICAO: BVR, Callsign: Bavarian, Designator 5P* recalled by IATA. | D | AVCODES |
| 76 | 5P | Panorama Airways Limited Liability Company | ICAO: UZP, Callsign: Panorama, | UZ | AVCODES |
| 77 | 5Q | Keenair Charter Ltd. | ICAO: JFK, Callsign: Keenair, | GB | AVCODES |
| 78 | 5Q | Euroceltic Airways Limited | ICAO: ECY, Callsign: Echelon, Ceased Operations on 21 January 2003. Designator 5Q reinstated by IATA. Name changed from Atlantic Bridge AviationLtd. d/b/a Sky Trek Airlines | GB | AVCODES |
| 79 | 5Q | Skargardsflyg AB | ICAO: LND, Callsign: Aaland, Designator 5Q recalled by IATA | FIN | AVCODES |
| 80 | 5Q | Atlantic Bridge AviationLtd. d/b/a Sky Trek Airlines | Name changed to Euroceltic Airways Limited | GB | AVCODES |
| 81 | 5Q | Best Aviation Ltd. | ICAO: BEA, Callsign: Best Air, Founded in 2007. Ceased operations in 2009. | BD | AVCODES |
| 82 | 5Q | Los Cipreses S.A. d/b/a BQB Lineas Aéreas | ICAO: BQB, IATA Prefix: 567, Suspened operations on 11 April 2015. Commenced operations in May 2010. Subsidiary of ferry company Buquebus. | ROU | AVCODES |
| 83 | 5Q | Holiday Europe Ltd. | ICAO: HES, Callsign: Holiday Europe, IATA Designator changed to 5Q. | BG | AVCODES |
| 84 | 5Q | Holiday Europe Ltd. | ICAO: HES, Callsign: Holiday Europe, IATA Prefix: 062, IATA Designator changed from 5Q*. | BG | AVCODES |
| 85 | 5R | Karthago Airlines | ICAO: KAJ, Callsign: Karthago, Founded in 2001. | TN | AVCODES |
| 86 | 5R | Rover Airways International, Inc. | ICAO: ROV, Callsign: Roverair, Designator 5R recalled by IATA | USA | AVCODES |
| 87 | 5R | IRS Aero, Joint-Stock Air Company | ICAO: LDF, Callsign: Elbrus, Designator 5R recalled by IATA | RUS | AVCODES |
| 88 | 5R | British Airways RTZ | IATA designator changed from QO*. Computer Reservation System | GB | AVCODES |
| 89 | 5R | Custom Air Transport, Inc. | ICAO: CTT, Callsign: Catt, Comenced operations 09 December 1995. | USA | AVCODES |
| 90 | 5R | Rutas Aereas, C.A. d/b/a RUTACA | ICAO: RUC, Callsign: Rutaca, Name changed to Rutas Aereas, C.A. d/b/a Rutaca Airlines | YV | AVCODES |
| 91 | 5R | Rutas Aereas, C.A. d/b/a Rutaca Airlines | ICAO: RUC, Callsign: Rutaca, Name changed from Rutas Aereas, C.A. d/b/a RUTACA | YV | AVCODES |
| 92 | 5S | Airspeed Aviation, Inc. | ICAO: SPD, Callsign: Speedline, Ceased operations | CDN | AVCODES |
| 93 | 5S | Servicios Aereos Profesionales, S.A. | ICAO: PSV, Callsign: Proservicios, IATA code changed from P2* | DOM | AVCODES |
| 94 | 5S | Société D’Exploitation Aeropostale | ICAO: ARP, Callsign: Aeroposte, Designator 5S recalled by IATA | F | AVCODES |
| 95 | 5S | Global Aviation Services Group | ICAO: GAK, IATA Prefix: 301, Designator 5S reinstated by IATA | LAR | AVCODES |
| 96 | 5T | Air Norterra Inc, d/b/a Canadian North | ICAO: MPE, Callsign: Emphis, IATA Prefix: 518, Founded in 1989. Name changed to Canadian North Inc. ICAO callsign changed to Empress. IATA designator changed from 3F* | CDN | AVCODES |
| 97 | 5T | Transwede Airways AB | ICAO: TWE, Callsign: Transwede, Founded in 2005. Ceased operations on 31 March 2010. Owned by Braathens Aviation. | S | AVCODES |
| 98 | 5T | Air Specialities, Inc. d/b/a Total Air | | USA | AVCODES |
| 99 | 5T | Transair Sweden AB | ICAO: TWE, Callsign: Trans-Swede, | S | AVCODES |
| 100 | 5T | Air Negril | Designator 5T recalled by IATA | JA | AVCODES |
