| 1 | 2A | Deutsche Bahn AG | ICAO: DBB, Railway Service | D | AVCODES |
| 2 | 2A | Deutsche Bahn AG | ICAO designator DBB cancelled. Railway Service | D | AVCODES |
| 3 | 2A | Astra Airways Limited d/b/a Air Astra | ICAO: AWA, | BD | AVCODES |
| 4 | 2B | ATA Aerocondor Transportes Aéreos Lda. | ICAO: ARD, Callsign: Aerocondor, IATA Prefix: 088, Suspended operation in May 2008. Designator 2B reinstated by IATA | P | AVCODES |
| 5 | 2B | Aerocondor Transportes Aereos Lda. | ICAO: ATA, IATA Prefix: 088, ICAO designator changed to ARD | P | AVCODES |
| 6 | 2B | Bahrain Air B.S.C. (Closed) | ICAO: BAB, Callsign: Awal, IATA Prefix: 937, IATA desigantor changed to BN | BRN | AVCODES |
| 7 | 2B | OJSC Ak Bars Aero | ICAO: BGM, Callsign: Bugavia, Ceased operations on 12 January 2015. Name changed from OJSC Bugulma Air Enterprise during 2010. | RUS | AVCODES |
| 8 | 2B | Albawings sh.p.k. | ICAO: AWT, Callsign: Albawings, | AL | AVCODES |
| 9 | 2C | SNCF | ICAO: TGV, Railway Service | F | AVCODES |
| 10 | 2C | SNCF | ICAO Designator TGV cancelled. Railway Service | F | AVCODES |
| 11 | 2C | CMA CGM Air Cargo S.A.S. | ICAO: CMA, Callsign: French Cargo, IATA Prefix: 003, | F | AVCODES |
| 12 | 2D | Aero VIP, S.A. | ICAO: AOG, Callsign: Avip, Designator 2D reinstated by IATA | RA | AVCODES |
| 13 | 2D | Demin Air | ICAO: DNM, IATA designator changed to 3D | NL | AVCODES |
| 14 | 2D | Federico II Airways, S.p.A. | ICAO: FDE, Callsign: Federeagle, Ceased operations. Designator 2D* recalled by IATA | I | AVCODES |
| 15 | 2D | Dubrovnik Airline d.o.o. | ICAO: DBK, Callsign: Seagull, Founded on 15 December 2004. Ceased operations on 23 October 2011. | HR | AVCODES |
| 16 | 2D | Joint Stock Company Airline Alania | ICAO: ALH, Callsign: White Swan, | RUS | AVCODES |
| 17 | 2D | Dynamic Aviation, LLC d/b/a Dynamic International Airways | ICAO: DYA, Callsign: Dynamic Air, IATA Prefix: 054, Name changed to Dynamic International Airways, LLC. Founded in 2010. | USA | AVCODES |
| 18 | 2D | Dynamic International Airways, LLC | ICAO: DYA, Callsign: Dynamic Air, IATA Prefix: 054, Name changed to Eastern Airlines, LLC w.e.f. 25 June 2018. Name changed from Dynamic Aviation, LLC d/b/a Dynamic International Airways. Founded in 2010. | USA | AVCODES |
| 19 | 2D | Eastern Airlines, LLC | ICAO: EAL, Callsign: Eastern, IATA Prefix: 054, Name changed from Dynamic International Airways, LLC w.e.f. 25 June 2018. Founded in 2010. | USA | AVCODES |
| 20 | 2D | Eastern Airlines, LLC | ICAO: EAL, Callsign: Eastern, IATA Prefix: 054, Name changed from Dynamic International Airways, LLC w.e.f. 25 June 2018. Founded in 2010. | USA | AVCODES |
| 21 | 2E | Sky Cabs (PVT) Ltd. | ICAO: SCB, Callsign: Sky Cabs, Designator 2E recalled by IATA | CL | AVCODES |
| 22 | 2E | Ireland Airways | ICAO: EIX, | IRL | AVCODES |
| 23 | 2E | Smokey Bay Air, Inc. | | USA | AVCODES |
| 24 | 2E | Tikal Jets, S.A. | ICAO: TKC, Callsign: Tikal, IATA designator changed to A5* | GCA | AVCODES |
| 25 | 2E | ave.com | ICAO: PHW, | UAE | AVCODES |
| 26 | 2F | Frontier Flying Service, Inc. | ICAO: FTA, Callsign: Frontier-Air, IATA Prefix: 517, Established in 1950. Rebranded as Ravn Connect during 2014. | USA | AVCODES |
| 27 | 2F | Payam (IPTAS) | ICAO: IRP, Callsign: Payamair, Designator 8P* recalled by IATA | IR | AVCODES |
| 28 | 2F | Afrinat International Airlines | | WAG | AVCODES |
| 29 | 2F | Fair Aviation (PTY) Ltd. t/a Fair Aviation | Designator 2F recalled by IATA. | ZA | AVCODES |
| 30 | 2F | twoFlex Taxi Aereo Ltda | ICAO: OWT, Callsign: Brasil Cargo, Name changed to Azul Conecta Ltda on 11 August 2020. ICAO designator changed to ACN. ICAO Callsign changed to Azul Conecta. Name changed from TWO Taxi Aereo Ltda. | BR | AVCODES |
| 31 | 2F | Frontier Flying Service, Inc. Branded as Ravn Connect | ICAO: FTA, Callsign: Frontier-Air, IATA Prefix: 517, Filed for Chapter 11 Bankruptcy, temporarily ceased all operations on 05 April 2020. Established in 1950. | USA | AVCODES |
| 32 | 2F | Azul Conecta Ltda | ICAO: ACN, Callsign: Azul Conecta, Name changed from twoFlex Taxi Aereo Ltda on 11 August 2020. ICAO designator changed from OWT. ICAO Callsign changed from Brasil Cargo. Name changed from TWO Taxi Aereo Ltda. | BR | AVCODES |
| 33 | 2G | Open Joint Stock Company "Angara Airlines" | ICAO: AGU, Callsign: Sarma, | RUS | AVCODES |
| 34 | 2G | Northwest Seaplanes, Inc. d/b/a San Juan Airlines | ICAO: MRR, Callsign: Mariner, Name changed to West Isle Air, Inc. d/b/a San Juan Airlines. Name changed from Northwest Seaplanes, Inc. | USA | AVCODES |
| 35 | 2G | Debonair Airways Ltd. | ICAO: DEB, Callsign: Debonair, Ceased operations. Designator 2G reinsgtated by IATA | GB | AVCODES |
| 36 | 2G | Northwest Seaplanes, Inc. | ICAO: MRR, Callsign: Mariner, Name changed to Northwest Seaplanes, Inc. d/b/a San Juan Airlines | USA | AVCODES |
| 37 | 2G | West Isle Air, Inc. d/b/a San Juan Airlines | ICAO: MRR, Callsign: Mariner, Name changed from Northwest Seaplanes, Inc. d/b/a San Juan Airlines. Previous name changed from Northwest Seaplanes, Inc. | USA | AVCODES |
| 38 | 2G | Cargoitalia S.p.A. | ICAO: CRG, IATA Prefix: 002, Founed in 2005. Ceased operations on 21 December 2011. | I | AVCODES |
| 39 | 2H | Thalys International | Designator 2H* reinstated by IATA. Railway Service | B | AVCODES |
| 40 | 2H | Hamsal Air Services Limited | ICAO: HML, | WAN | AVCODES |
| 41 | 2I | Star Up, S.A. | ICAO: SRU, Callsign: Star-up, Name changed to Star Up S.A. dba Star Peru | PE | AVCODES |
| 42 | 2I | Star Up S.A. dba Star Peru | ICAO: SRU, Callsign: Star-up, IATA Prefix: 156, Name changed from Star Up, S.A. | PE | AVCODES |
| 43 | 2I | 21 Air LLC | ICAO: CSB, Callsign: Cargo South, IATA Prefix: 681, Founded on 25 February 2014. | USA | AVCODES |
| 44 | 2J | Air Burkina | ICAO: VBW, Callsign: Burkina, IATA designator changed from VH | BF | AVCODES |
| 45 | 2J | China Cargo Airlines Ltd. | ICAO: CHY, IATA designator changed to CK. ICAO designator changed to CKK. | PRC | AVCODES |
| 46 | 2K | Aerolineas Galapagos S.A. AeroGal | ICAO: GLG, Callsign: Aerogal, Name changed to Avianca Ecuador w.e.f. 18 June 2014. IATA Designator changed from 2K* | EC | AVCODES |
| 47 | 2K | Aerolineas Galapagos S.A. AeroGal | ICAO: GLG, Callsign: Aerogal, IATA Designator changed to 2K | EC | AVCODES |
| 48 | 2K | KLM (DCS) | Designator 2K* recalled by IATA. Departure Control System | NL | AVCODES |
| 49 | 2K | Kitty Hawk Airways d/b/a 135 Airways, Inc. | ICAO: KHA, Name changed to Kitty Hawk Air Cargo, Inc. | USA | AVCODES |
| 50 | 2K | Avianca Ecuador d/b/a Avianca | ICAO: GLG, Callsign: Galapagos, Name changed from Aerolineas Galapagos S.A. AeroGal w.e.f. 18 June 2014. IATA Designator changed from 2K* | EC | AVCODES |
| 51 | 2L | Lineas Aereas Entre Rios Sociedad Del Estado | Designator 2L reinstated by IATA | RA | AVCODES |
| 52 | 2L | Transportes Aereos Bolivianos (TAB) | ICAO: BOL, Callsign: Bol, IATA Prefix: 901, | BOL | AVCODES |
| 53 | 2L | Helvetic Airways AG | ICAO: OAW, Callsign: Arabella, ICAO Callsign changed to Helvetic. Name changed from Odette Airways AG. | CH | AVCODES |
| 54 | 2L | Airblue Limited | ICAO: ABQ, Callsign: Pakblue, IATA designator changed to ED | PK | AVCODES |
| 55 | 2L | Helvetic Airways AG | ICAO: OAW, Callsign: Helvetic, ICAO Callsign changed from Arabella. Name changed from Odette Airways AG. | CH | AVCODES |
| 56 | 2M | Moldavian Airlines | ICAO: MDV, Callsign: Moldavian, IATA Prefix: 860, Established on 26 July 1994. Commenced operations on 19 August 1994. Ceased operations during 2014. | MD | AVCODES |
| 57 | 2M | Maya Island Air | ICAO: MYD, Callsign: Myland, IATA designator changed from MY | BH | AVCODES |
| 58 | 2N | Haiti Aviation S.A. d/b/a D'Ayiti | Designator 2N recalled by IATA | RH | AVCODES |
| 59 | 2N | Starwelt Trans Lloyd Cargo Ltd. | | MW | AVCODES |
| 60 | 2N | Starwelt Trans Lloyd Cargo Ltd. d/b/a Starwelt Airways | | ZA | AVCODES |
| 61 | 2N | National Air Services | ICAO: KNE, IATA designator changed to XY* | SA | AVCODES |
| 62 | 2N | NEX Time Jet AB | ICAO: NTJ, Callsign: Nextjet, Name changed to NextJet AB | S | AVCODES |
| 63 | 2N | NextJet AB | ICAO: NTJ, Callsign: Nextjet, Suspended all operations and filed for bankruptcy on 16 May 2018. Name changed from NEX Time Jet AB | S | AVCODES |
| 64 | 2N | Aviation Transport Company Yuzmashavia | ICAO: UMK, Callsign: Yuzmash, | UA | AVCODES |
| 65 | 2N | SmartLynx Airlines Malta Limited | ICAO: LYX, Callsign: Malta Cat, Designator 2N recalled by IATA | M | AVCODES |
| 66 | 2O | Air Salone | ICAO: RNE, | WAL | AVCODES |
| 67 | 2P | Air Philippines Corporation | ICAO: GAP, Callsign: Orient Pacific, IATA Prefix: 211, ICAO callsign changed to Airphil Express w.e.f. 10 April 2008. Designator 2P reinstated by IATA. IATA designator changed from 3G* | RP | AVCODES |
| 68 | 2P | Prairie Flying Service (1976) Ltd. | ICAO: PFS, Callsign: Prairie, Designator recalled by IATA | CDN | AVCODES |
| 69 | 2P | Air Philippines Corporation | ICAO: APQ, ICAO designator changed to GAP. IATA designator changed from 3G* | RP | AVCODES |
| 70 | 2P | Puerto Rico Air Management Service Inc | IATA Prefix: 902, Designator 2P* restated by IATA | PRI | AVCODES |
| 71 | 2P | Air Philippines Corporation d/b/a Airphil Express | ICAO: GAP, Callsign: Airphil, IATA Prefix: 211, Name changed to Air Philippines Corporation d/b/a PAL Express and Airphil Express. IATA designator changed from 3G*. ICAO callsign changed from Orient Pacific. Designator 2P reinstated by IATA. | RP | AVCODES |
| 72 | 2P | Air Philippines Corporation d/b/a PAL Express and Airphil Express | ICAO: GAP, Callsign: Airphil, IATA Prefix: 211, Name changed from Air Philippines Corporation. W.e.f. 15 March 2013. IATA designator changed from 3G*. ICAO callsign changed from Orient Pacific. Designator 2P reinstated by IATA | RP | AVCODES |
| 73 | 2Q | Air Cargo Carriers, Inc. | ICAO: SNC, Callsign: Night Cargo, IATA Prefix: 883, Designator 2Q reinstated by IATA. IATA designator changed from 3U* | USA | AVCODES |
| 74 | 2Q | International Aviatransport Corporation Rusavia | | RUS | AVCODES |
| 75 | 2Q | Millenium Air Corporation d/b/a Millair | | I | AVCODES |
| 76 | 2Q | Pinair Panamanian International Airlines | Designator 2Q recalled by IATA | PA | AVCODES |
| 77 | 2Q | Avitrans Nordic AB | ICAO: ETS, | S | AVCODES |
| 78 | 2Q | Air Cargo Carriers, LLC. | ICAO: SNC, Callsign: Night Cargo, Designator 2Q reinstated by IATA. IATA designator changed from 3U* | USA | AVCODES |
| 79 | 2Q | Nuovo Trasporto Viaggiatori S.p.A. | Designator 2Q* recalled by IATA. Railway Service | I | AVCODES |
| 80 | 2R | STAR Airlines | ICAO: SEU, Callsign: Starway, IATA Prefix: 473, IATA Designator changed to SE. Name changed from STAR Europe (Societe de Transport Aerien Regional) | F | AVCODES |
| 81 | 2R | VIA Rail Canada, Inc. | ICAO: VRR, Accounting Code 830 recalled by IATA. Railway Service | CDN | AVCODES |
| 82 | 2R | STAR Europe (Société de Transport Aerien Regional) | ICAO: SEU, Callsign: Starway, IATA Prefix: 473, Name changed to STAR Airlines | F | AVCODES |
| 83 | 2R | VIA Rail Canada, Inc. | ICAO designator VRR cancelled. Accounting Code 830 recalled by IATA. Railway Service | CDN | AVCODES |
| 84 | 2R | Sunlight Express Airways t/a Sunlight Air | ICAO: RLB, Callsign: Sunlight, | RP | AVCODES |
| 85 | 2S | Island Express | ICAO: SDY, Callsign: Sandy Isle, IATA Prefix: 579, | USA | AVCODES |
| 86 | 2S | Sunflower Airlines Ltd. | ICAO: SUF, Callsign: Sunflower, IATA designator changed to PI | FJI | AVCODES |
| 87 | 2S | Satgur Air Transport | Designator 2S* recalled by IATA | LB | AVCODES |
| 88 | 2S | Star Equatorial Airlines | IATA Prefix: 315, | GUI | AVCODES |
| 89 | 2S | Kinda Airlines | ICAO: KJA, Callsign: Kinda, IATA Prefix: 338, Never commenced operations. | SYR | AVCODES |
| 90 | 2S | Air Carnival Private Limited | ICAO: ACV, Callsign: Carnair, | IND | AVCODES |
| 91 | 2S | CA Air Stork SRL | ICAO: MSB, Callsign: Long, IATA designator changed from 0S*. | MD | AVCODES |
| 92 | 2S | Southwind Airlines | ICAO: STW, Callsign: Moon Star, Established by tour operator Pegas Touristik. | TR | AVCODES |
| 93 | 2T | Canada 3000 Airlines Limited | ICAO: CMM, Callsign: Elite, Ceased operations on 08 November 2001. Name changed from Air 2000 Canada Airlines Ltd. In May 1989. | CDN | AVCODES |
| 94 | 2T | Tulpar Air Service | ICAO: TUX, Callsign: Tulpa, Designator 2T recalled by IATA | KZ | AVCODES |
| 95 | 2T | Haiti Ambassador Airlines | ICAO: HAM, Founded in April 2002. Commenced operations in 2003. | RH | AVCODES |
| 96 | 2T | Turbo Mega Airways Pvt. Ltd. t/a trujet | ICAO: TRJ, Callsign: Trujet, Suspended operations on 15 February 2022. | IND | AVCODES |
| 97 | 2T | Kenya homes Limited d/b/a Timbis Air Services | ICAO: TBS, Callsign: Timbis, IATA Prefix code 212 recalled. | EAK | AVCODES |
| 98 | 2T | Air 2000 Canada Airlines Ltd. | ICAO: CMM, Callsign: Elite, Name changed from to Canada 3000 Airlines Limited in May 1989. | CDN | AVCODES |
| 99 | 2U | Sunair Express LLC | Ceased operations on 29 September 2001. Designator 2U recalled by IATA | USA | AVCODES |
| 100 | 2U | Karat Joint Stock Company | ICAO: AKT, Callsign: Aviakarat, IATA designator changed to V2 | RUS | AVCODES |
