| 1 | 2U | Sunair Express LLC | Ceased operations on 29 September 2001. Designator 2U recalled by IATA | USA | AVCODES |
| 2 | 2U | Karat Joint Stock Company | ICAO: AKT, Callsign: Aviakarat, IATA designator changed to V2 | RUS | AVCODES |
| 3 | 2U | Air Guinee Express S.A. | ICAO: GIP, Callsign: Future Express, IATA Prefix: 454, | GUI | AVCODES |
| 4 | 2U | Atlasjet Uuslararasi Havacilik A/S | ICAO: OGE, Callsign: Atlasjet, IATA designator changed to KK. Name changed from Atlas International Airlines - Atlas International Havacilik A/S | TR | AVCODES |
| 5 | 2U | Sun d'Or International Airlines | ICAO: ERO, Callsign: Echo Romeo, As of March 2013, all Sun d'Or's flights are operated by El Al under Sun d'Or, Arkia and Israir brand. Authorities revoked operating licence. Ceased operations 01 April 2011. Designator 2U restated/changed by IATA | IL | AVCODES |
