| 501 | EBEL | EBEL TRANSFER INC | | | SCAC4 |
| 502 | EBER | EBERLY, G E & SON INC | | | SCAC4 |
| 503 | EBET | EBERLE TRUCKING | | | SCAC3 |
| 504 | EBF | MSR Flug-Charter GmbH | Callsign: Skyrunner, Ceased operations on 31 October 2008. | D | AVCODES |
| 505 | EBF | Echo Airlines Ltd. | | BS | AVCODES |
| 506 | EBF | MSR Flug-Charter | | | ICAO1 |
| 507 | EBFC | EBERT FERTILIZER COMPANY | | | SCAC3 |
| 508 | EBFL | Verein der Eisenbahnfreunde in Lienz | | AT | VKM |
| 509 | EBFT | EARLY BIRD FREIGHT LINES INC | | | SCAC3 |
| 510 | EBG | Eurosense | Callsign: Eurosense, Ceased Operations. | BG | AVCODES |
| 511 | EBG | Eurosense | | | ICAO1 |
| 512 | EBG | Eisenbahn-Betriebs-Gesellschaft mbH | Altenbeken | D | privat-bahn.de |
| 513 | EBG | | | D | TRAFFIC |
| 514 | EBG | Eisenbahn-Betriebsgesellschaft | | DE | VKM |
| 515 | EBGI | E B TRUCKING INC | | | SCAC3 |
| 516 | EBGL | EMBRYO GENETICS LTD | | | SCAC4 |
| 517 | EBGO | Eisenbahnbetriebsgesellschaft Oberelbe | | D | TRAFFIC |
| 518 | EBGR | Eastern Berks Gateway Railroad | | | RSRV |
| 519 | EBGX | Electro Bleaching Gas Co. | 8/1920-1/1941 | | NAKINA |
| 520 | EBGX | Electro Bleaching Gas Co. | Apr-38 | | NRHS |
| 521 | EBGX | ELECTRO BLEACHING GAS CO. | | | PWRC |
| 522 | EBHI | EISS BROTHERS INC | | | SCAC4 |
| 523 | EBIN | ERNIE BALL INC | | | SCAC4 |
| 524 | EBIV | EMERICK BROS INC | | | SCAC4 |
| 525 | EBJ | European Business Jets plc | Callsign: Sky Elite, | GB | AVCODES |
| 526 | EBJ | European Business Jets | | | ICAO1 |
| 527 | EBJM | EDMONDS, BILLY JIM | | | SCAC3 |
| 528 | EBJM | ESSBERGER, JOHN T GMBH & CO KG | | | SCAC4 |
| 529 | EBKC | EDINBURGH BULK CARRIERS LIMITED | | | SCAC3 |
| 530 | EBKE | EXECUTIVE TRANSPORTATION AND BROKER | | | SCAC4 |
| 531 | EBKL | EDELBROCK LLC | | | SCAC4 |
| 532 | EBKO | ELLENBECKER OIL INC | | | SCAC4 |
| 533 | EBKQ | EUBANK, DON | | | SCAC3 |
| 534 | EBL | EAST BRANCH & LINCOLN | | | PWRC |
| 535 | EBLE | EBLE, GEORGE M JR | | | SCAC3 |
| 536 | EBLE | ESTES BROKERAGE LLC | | | SCAC4 |
| 537 | EBLG | EASTON, BRUCE LEASING LTD | | | SCAC3 |
| 538 | EBLI | ESCOT BUS LINES INC | | | SCAC4 |
| 539 | EBLK | EVANS, B L TRUCKING INC | | | SCAC3 |
| 540 | EBLL | ESTAIRE BUS LINES | | | SCAC3 |
| 541 | EBLN | EPPS, B L TRUCKING | | | SCAC4 |
| 542 | EBLQ | ENNABE LOGISTICS INC | | | SCAC4 |
| 543 | EBLR | EDDIE BOY & LEE ROY TRANSPORTATION | | | SCAC3 |
| 544 | EBLR | EARLY BLOOM INC | | | SCAC4 |
| 545 | EBLS | ELITE BUS LINES LTD | | | SCAC3 |
| 546 | EBLS | E BIOFUELS LLC | | | SCAC4 |
| 547 | EBLT | EIGHT BALL LINE TRUCKING | | | SCAC3 |
| 548 | EBLU | EVERBRIDGE SHIPPING LLC | | UAE | BIC |
| 549 | EBLU | EUROBRIDGE LINES | | | SCAC3 |
| 550 | EBM | Eisenbahnbetriebsgesellschaft Mittelrhein mbH | Gummersbach | D | privat-bahn.de |
| 551 | EBM | Eisenbahn-Verkehrsgesellschaft im Bergisch-Märkischen Raum mbH | Dieringhausen | D | privat-bahn.de |
| 552 | EBM | EBM Cargo | | D | TRAFFIC |
| 553 | EBM | EBM Cargo GmbH | | DE | VKM |
| 554 | EBMQ | EARLY BIRD MOTOR EXPRESS INC | | | SCAC3 |
| 555 | EBN | Eisenbahnnetzwerk Bremen/Niedersachsen | Bremen | D | privat-bahn.de |
| 556 | EBN | Eisenbahnbedarf Bad Nauheim | | DE | VKM |
| 557 | EBNI | EARTHBORNE INC | | | SCAC4 |
| 558 | EBNS | EDDIES BAKE-N-SERVE INC | | | SCAC3 |
| 559 | EBNV | EBONY COMPANIA NAVIERA SA | | | SCAC3 |
| 560 | EBNY | EBONY TRUCKING | | | SCAC3 |
| 561 | EBO | Eisenbahnbedarf Bad Orb | Bad Orb | D | privat-bahn.de |
| 562 | EBO | Eisenbahn-Betriebsgesellschaft Ochsenhausen GmbH | Ochsenhausen | D | privat-bahn.de |
| 563 | EBOC | ESPEY, BILLY OIL CO INC | | | SCAC4 |
| 564 | EBOL | EBRO LLC | | | SCAC4 |
| 565 | EBOT | EDWARDS BROS TRUCKING INC | | | SCAC3 |
| 566 | EBPL | EBENISTERI PLOURDE | | | SCAC4 |
| 567 | EBPR | EBPRODUCE | | | SCAC4 |
| 568 | EBPT | EAGLE BULK PTE LTD | | | SCAC4 |
| 569 | EBPX | Empire Builder Private Cars | 7/2000-7/2005 (not listed) | | NAKINA |
| 570 | EBPX | Empire Builder Private Cars | Jul-00 | | NRHS |
| 571 | EBPX | EMPIRE BUILDER PRIVATE CARS | | | PWRC |
| 572 | EBPX | Empire Builder Private Cars | | | RSRV |
| 573 | EBPX | EMPIRE BUILDER PRIVATE CARS | | | SCAC4 |
| 574 | EBQE | EMBARQUE CAMDEN | | | SCAC4 |
| 575 | EBR | Eisenbahnfreunde Breisgau | | D | TRAFFIC |
| 576 | EBRD | EUROPEAN BREADS | | | SCAC4 |
| 577 | EBRL | ECKELS, B & R TRANSPORT LTD | | | SCAC3 |
| 578 | EBRN | EABORN TRUCK SERVICE, INC | | | SCAC3 |
| 579 | EBRO | EALY BROS TRUCKING COMPANY | | | SCAC3 |
| 580 | EBRO | ECONOMY BROKERAGE LLC | | | SCAC4 |
| 581 | EBS | Eli-fly S.p.A. | Callsign: Elifly, | I | AVCODES |
| 582 | EBS | AEG Aviation Services | | USA | AVCODES |
| 583 | EBS | Erfurter Bahnservice GmbH | Erfurt | D | privat-bahn.de |
| 584 | EBS | Erfurter Bahnservice GmbH | | D | TRAFFIC |
| 585 | EBS | Erfurter Bahnservice GmbH | | DE | VKM |
| 586 | EBSB | E B SPARS INC | | | SCAC4 |
| 587 | EBSC | EFFKAY BUS CO INC | | | SCAC3 |
| 588 | EBSC | EIGHT B'S LLC | | | SCAC4 |
| 589 | EBSL | ERWIN BROS SPECIALIZED LLC | | | SCAC4 |
| 590 | EBSR | East Brookfield & Spencer R.R. LLC | 7/2005 (not listed) | | NAKINA |
| 591 | EBSR | EAST BROOKFIELD & SPENCER RAILROAD, LLC | | | PWRC |
| 592 | EBSR | EAST BROOKFIELD & SPENCER RAILROAD | | | SCAC4 |
| 593 | EBST | EBS TRANSPORT INC | | | SCAC4 |
| 594 | EBSV | ELLIOTT BULK SERVICES LLC | | | SCAC4 |
| 595 | EBT | EAST BROADTOP RAILROAD & COAL CO. | | | PWRC |
| 596 | EBT | Emmental - Burgdorf - Thun-Bahn | | CH | TRAFFIC |
| 597 | EBT | EBT - EMONS Bahntransporte GmbH | | DE | VKM |
| 598 | EBTB | EDDIE BRAVO TRANSPORT LLC | | | SCAC4 |
| 599 | EBTD | EBY - BROWN TRANSPORTATION LLC | | | SCAC4 |
| 600 | EBTF | EAGLEONE BULK TRANSPORTATION LLC | | | SCAC4 |
